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NGS Data Analysis and Integration with Microarray Data in Partek Flow

The NIH Library Bioinformatics Support Program has organized a hands-on class where students will learn RNA-Seq and DNA-Seq data analysis, and how to integrate NGS data with microarray data using Partek Flow. This class will be held on April 6, from 9:30 a.m. to 4:00 p.m., and will consist of two parts. The morning session will be devoted to analysis using a sample dataset. This will be followed by an open lab where researchers can bring their own data for analysis.

Students will learn to use the following features of Partek Flow:

  • Import data in FASTQ format
  • Perform QA/AC
  • Alignment
  • Gene/transcript abundance estimate
  • Differential Expression Detection
  • Filter gene list
  • GO Enrichment Analysis
  • Variant Detection
  • Variant annotation and filtering
  • Visualization
  • Quality score distribution
  • Base composition
  • PCA scatterplot
  • Dotplot
  • Hierarchical clustering
  • Chromosome view

Integration of RNA-Seq with Microarray Gene Expression Data

In addition, students will learn how to overcome common analysis challenges.

This class will be taught by Partek staff and is free and open to NIH staff.

Access to the NIH Library license for Partek Flow requires two accounts. 1) Obtain a Helix Systems Account by following these instructions https://hpc.nih.gov/docs/accounts.html. 2) Email your Helix account login name to Dr. Lynn Young (lynny@mail.nih.gov) to register for the Partek Flow account.

Registration for this class is closed.

The NIH Library in Building 10 serves the information needs of NIH staff and select Department of Health and Human Services (HHS) agencies. The NIH Library is part of the Office of Research Services (ORS) in the Office of the Director (OD).