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October 6 – 8: Partek User Group Meeting and Microarray & NGS Data Analysis Class

Partek will hold a user group meeting on October 6 from 9 a.m. to 1 p.m.

The NIH Library Bioinformatics Support Program will host the Partek: Microarray and NGS Data Analysis class October 7 (9 a.m. – 3 p.m.) and October 8 (9 a.m. – 4 p.m.). The free training will be held in the NIH Library Training Room, Building 10. This is a two-day class. Day 1 will cover Partek Genomics Suite; Day 2 will cover Partek Flow. Registration is required (use the link provided below).

Students will learn how to use basic features of Partek Genomics Suite and Partek Flow on microarray and NGS data.

Partek Genomics Suite (PGS)

  • Import data
  • Perform QA/QC of imported data
  • Detect differential expression (ANOVA)
  • Create a gene list
  • Interpret biological information
  • Implement visualization (PCA, histogram, box plot, dot plot, volcano plot, interaction plot, heatmap)

Partek Flow

  • Import data
  • Perform QA/AC
  • Use alignment
  • Estimate gene/transcript abundance
  • Detect differential expression
  • Filter gene list
  • Perform GO enrichment analysis
  • Use variant detection
  • Use variant annotation and filter
  • Conduct Seqduo analysis
  • Implement visualization

Day 1: The class will start with hands-on training on analysis of expression microarray data in PGS. Affymetrix miRNA data will be used to generate an miRNA expression workflow: data import, QA/QC, exploratory data analysis will be discussed, followed by differential expression detection analysis and significant gene list creation. Partek Pathway will be demonstrated on biological interpretation.

Day 2: Hands-on RNA-Seq and DNA-Seq training on Partek Flow. It starts from importing raw sequence data in fastq format. Since there will be an open lab in the afternoon, you may bring in your own data for analysis.

This class will be taught by Partek research staff and is open to NIH staff.

For more information on how to access the NIH Library's licensed Partek site, please visit https://nihlibrary.nih.gov/services/bioinformatics-support/analysis-tools-databases.

Sign language interpreters and other reasonable accommodations can be provided. If you require these accommodations, please contact the NIH Library Information Desk at 301.496.1080 five business days in advance. For TDD users/callers, please call the above number through the Federal Relay Service at 1.800.877.8339.

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