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Register Now for Partek Flow Class: NGS Data Analysis

Bioinformatics

Tuesday, August 30, 2016
9:30 a.m. – 4:00 p.m.
NIH Library Training Room, Building 10
Registration for this class is no longer available.

The NIH Library Bioinformatics Support Program has organized a hands-on class in which participants will learn about RNA-Seq and DNA-Seq data analysis and how to integrate NGS data with microarray data using Partek Flow. This two-part class will be held on August 30, from 9:30 a.m. to 4:00 p.m. The morning session will be devoted to analysis using a sample dataset. For the afternoon session, which is designed as an open lab, researchers are encouraged to bring their own data for analysis.

Participants will learn to use the following features of Partek Flow:

  • Data import in FASTQ format
  • QA/QC
  • Alignment
  • Gene/transcript abundance estimate
  • Differential Expression Detection
  • Gene list filtering
  • GO enrichment analysis
  • Variant detection
  • Variant annotation and filtering
  • Visualization
    • Quality score distribution
    • Base composition
    • PCA scatterplot
    • Dotplot
    • Hierarchical clustering
    • Chromosome view
  • Integration of RNA-Seq with Microarray Gene Expression Data

This class will be taught by Partek staff and is free and open to NIH staff.

Access to the NIH Library license for Partek Flow requires two accounts, to be registered as follows:

  1. Obtain a High Performance Computing (HPC) Helix account: https://hpc.nih.gov/docs/accounts.html
  2. Email your HPC Helix account login name to Dr. Lynn Young (lynny@mail.nih.gov) to register for a Partek Flow account

Registration for this class is no longer available.

Sign language interpreters and other reasonable accommodations can be provided. If you require such accommodations, please contact the NIH Library Information Desk at 301.496.1080 five business days in advance. For TDD users/callers, please call the above number through the Federal Relay Service at 1.800.877.8339.

The NIH Library in Building 10 serves the information needs of NIH staff and select Department of Health and Human Services (HHS) agencies. The NIH Library is part of the Office of Research Services (ORS) in the Office of the Director (OD).

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