Partek Flow: Hands-On RNA-Seq Data Analysis

  • Registration Closed
  • May 15, 2018
  • 09:30 AM to 12:00 PM
  • NIH Library Training Room

Bioinformatics is an interdisciplinary field that develops methods and software tools for understanding biological data. As an interdisciplinary field of science, bioinformatics combines computer science, statistics, mathematics, and engineering to analyze and interpret biological data.

The NIH Library provides access to many analysis tools and databases on the bioinformatics workstations. Reservations are required to use the workstations.

Session Description

Abstract: Students will learn how to use the point-and-click interface in Partek Flow to go from raw data to experimental results for RNA-Seq analysis:

- Import data from .fastq files

- Perform QA/QC (Pre-alignment QA/QC, Post-alignment QA/QC)

- Trim bases

- Align reads to reference genome

- Quantify gene/transcript abundance

- Normalize gene counts

- Detect differentially expressed genes

- Filter a gene list

- Identify enriched KEGG pathway and/or GO terms

- Visualization:

  • Heat maps
  • Volcano plots
  • PCA scatterplot
  • Dot plots
  • Hierarchical clustering
  • Chromosome view
  • And more

Benefits: Acquire working knowledge of tools available to NIH researchers for the start to finish RNA-Seq data analysis

Description: Hands-on RNA-Seq Data Analysis training on Partek Flow.

Who should attend: NIH staff interested in learning RNA-Seq data analysis.

Time Required: 2.5 hours

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